Antibacterial Activity of Alcoholic and Aqueous Extracts of Various Organs of Citrus medica on 10 Human Pathogenic in Vitro

Background and Aims: Medicinal herbs are used due to fewer side effects for producing natural drugs in the pharmaceutical industry. The aim of this study was investigation of antibacterial and antioxidant activity of Citrus medica skin extracts against 10 human pathogenic bacterial and investigation of secondary metabolites in vitro. Materials and Methods: The colored and white part of the skin of Citrus medica under the supervision of Citrus Research Center experts were collected from Ramsar city, Mazandaran Province, Iran in 2014. Antibacterial activity by agar well diffusion method, total phenolic content by Folin-Ciocalteu method, total flavonoid by aluminum chloride and antioxidant properties by DPPH method were investigated. Phytochemical investigation of secondary metabolites was performed. Results and Conclusion: The highest inhibitory activity was observed on methanol extract of color skin on Bacillus cereus bacteria. IC50 methanol extract of color, white skin and ascorbic acid were calculated 0.1505, 0.1738 and 0.1095 mg/mL respectively. The total phenolic content of colored and white part of the skin was 109.5 and 105.6 (mgGAE/g) and the total flavonoid content was 3.53 and 3.02 (mgQ/g), respectively. The presence of alkaloid in methanol extract of the colored part and the presence of saponin and tannin in methanol extract of the white part was confirmed. Due to its antibacterial properties, this medicinal plant can be used in producing antibiotics, controlling human pathogenic microorganisms.